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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but in-depth nucleotide sequence data isn't still obtainable.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.

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This information outlines even more feasible optimization of mobile and immunoassay parameters to be certain the absolute best final results are...

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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best results are...

Antibodies bound to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.

Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which lead to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...

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